منابع مشابه
The Use of Potassium Permanganate as an Electron-Dense Stain for Sections of Tissue Embedded in Epoxy Resin
Potassium permanganate has become an established fixative in electron microscopy (Luft, 1956) and has special value in the study of cell membranes. During an investigation of methods of increasing the contrast in the electron microscope of tissues embedded in epoxy resin, t a solution of potassium permanganate was used to treat the sections, using a technique similar to that of Watson (1958). P...
متن کاملOrcein as a mucin stain for gastrointestinal tissue sections.
Although orcein has been in use for a long time as a stain for elastic fibres, it is only recently that it has been rediscovered for the demonstration of a variety of other substances. These include hepatitis B surface antigen (HBsAg),' 2 copper-associated protein,34 and more recently, intestinal sulphated mucins.35 Sipponen5 studied the normal distribution of orceinpositive mucin in the gastro...
متن کاملOxidized p-phenylenediamine staining of epoxy resin sections.
Semithin and thin sections of glutaraldehyde-fixed, epoxy resin-embedded animal tissues were treated with solutions of oxidized p-phenylenediamine (PPD). This method is suitable to reveal the general morphology of tissues in light microscopy, showing a high staining degree in some polyanion containing components. Posttreatments of thin sections with gold chloride solutions give considerable ele...
متن کاملPreparation of thin epoxy resin sections from thick sections of paraffin-embedded material.
PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...
متن کاملBiodeterioration of Polychrome Roman Mosaics
The mosaics from rhe Roman remains ollralica. which was lounded in 206 Be near presenr·day Seville. are rhrearened by rhe raval(es ol narure rhar are desrroyinl( rhese masrerpieces from rhe second cenrury AD. Since rhe heginninl( ofrhis century. rhe exposure ofmosr olrhe mosaics a.fier excavarion has resulred in colonizarion by pioneering species ol lichens and mosses. as well as by subsequent ...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1997
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500061642